Journal: Biology of reproduction
Article Title: The activin-follistatin system in the neonatal ovine uterus.
doi: 10.1095/biolreprod.103.016287
Figure Lengend Snippet: FIG. 2. Expression of ActRs in the neonatal ovine uterus. In situ hybridization and immunohistochemical analysis of ActRIA (A) and ActRII (B) and immunohistochemical analysis of ActRIB (C) in the uterus. Except for ActRIB, representative photomicrographs of in situ hybridization results are presented in bright-field and dark-field illumination (left). Melanocytes (Mel) in the endometrium appear white in the dark-field images and black in bright-field images, but they do not express subunit mRNA. Representative photomicrographs of immunohistochemical results are presented for the upper and lower portions of the uterine wall (right). As a negative control, mouse IgG (mIgG) was substituted for the primary antibody. M, Myometrium; S, stroma. Bars 5 50 mm.
Article Snippet: Mouse anti-human monoclonal antibody to follistatin (catalog no. MAB669), ActRIA (catalog no. MAB637), ActRIB (catalog no. MAB222), and ActRIIA/B (catalog no. MAB3391) were from R&D Systems, Inc. (Minneapolis, MN).
Techniques: Expressing, In Situ Hybridization, Immunohistochemical staining, Negative Control